- Hans-Adam II, Prince of Liechtenstein (1945)
- Princess Marie Aglaë of Liechtenstein Countess von Wchinitz and Tettau (1940, m. 1967)
- Hereditary Prince Alois (1968)
- Hereditary Princess Sophie, Duchess in Bavaria (1967, m. 1993)
- Prince Joseph Wenzel of Liechtenstein, Count of Rietberg (1995)
- Princess Marie-Caroline of Liechtenstein, Countess of Rietberg (1996)
- Prince Georg of Liechtenstein, Count of Rietberg (1999)
- Prince Nikolaus of Liechtenstein, Count of Rietberg (2000)
- Prince Maximilian Nikolaus Maria of Liechtenstein (1969)
- Prince Constantin Ferdinand Maria of Liechtenstein (1972)
- Princess Marie Gabriele of Liechtenstein, Countess von Kálnoky de Kőröspatak (1975, m. 1999)
- Princess Tatjana of Liechtenstein (1973)
- Baron Matthias Claus-Just Carl Philipp von Lattorff (1968, m. 1999)
- Prince Philipp Erasmus of Liechtenstein (1946)
- Isabelle Fernande Ghislaine Guillemette Elisabeth de L'Arbre de Malander (1947, m. 1971),
- Prince Alexander Wilhelm Hans Adam of Liechtenstein (1972)
- Astrid Barbara Kohl (1968, m. 2003)
- Princess Theodora of Liechtenstein (2004)
- Prince Wenzeslaus of Liechtenstein (1974)
- Prince Rudolf Ferdinand of Liechtenstein (1975).
- Miss Tilsim Tanberk (m. 2012)
- Princess Alienor Faye of Liechtenstein (2014-2015)
- Princess Nora of Liechtenstein (1950)
- Vicente Sartorius y Cabeza de Vaca, 3rd Marqués de Mariño (1931-2002, m. 1988)
- Doña María Teresa Sartorius y de Liechtenstein (1992)
Prince Nikolaus was made a Knight of Justice in the minority of the Sovereign Military Order of Malta at the age of 3. However, the class of knights in the minority was abolished in 1961, he became a Knight of Honor and Devotion in the Order.
He was educated in Switzerland. He attended his primary education in Vaduz before attended Schottengymnasium and Lyceum Alpinum Zuoz. From 1968-1972, he studied law at the University of Vienna, graduating with a Doctor Juris.
For a year after graduation, Prince Nikolaus worked as a Wissenschaftlicher Assistent at the International Committee of the Red Cross. For a year after that, he worked for courts in Vaduz. From 1977-1978, he was an advisor to the Office of International Relations of the Liechtenstein government. In 1979, he began a ten year tenure as a Permanent Representative of Liechtenstein to the Council of Europe. In 1986, he was appointed as a non-resident Ambassador of Liechtenstein to the Holy See, a position he holds still 30 years later. From 1989 to 1996 he was Ambassador of Liechtenstein to Switzerland. From 1996 to 2010 he was Ambassador of Liechtenstein to Belgium.
In March 1982, he married Princess Margaretha of Luxembourg. Together, they have four children:
- Prince Leopold Emmanuel Jean Marie of Liechtenstein (1984-1984)
- Princess Maria-Anunciata Astrid Joséphine Veronica of Liechtenstein (1985)
- Princess Marie-Astrid Nora Margarita Veronica of Liechtenstein (1987)
- Prince Josef-Emanuel Leopold Marie of Liechtenstein (1989)